3,3',5,5'-Tetramethylbenzidine (TMB) membrane peroxidase substrate Plus, liquid, Biotechnology Grade 1 component
Supplier: VWR
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Warning
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Synonyms:
TMB
, 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine
Ready to use sensitive substrate for the detection of horseradish peroxidase (HRP) activity suitable for ELISA and solution assays. Offers faster signal, enhanced sensitivity and the lowest possible background.
- Ready to use solution
Reaction of the substrate with HRP generates a free-radical cation that exhibits blue colour with an absorption maximum at 653 nm. Acidification of the reaction by addition of sulfuric acid yields a yellow-coloured diimine terminal oxidation product, which absorbs light at 450 nm.
TMB Plus liquid component substrate is optimised with TMB and hydrogen peroxide concentrations that allow a linear response to typical HRP concentrations utilised in immunologic assays.
Ordering information: Contains 2,08 mmol/l TMB, hydrogen peroxide, citric acid buffer pH 3,3 and additional proprietary, non toxic stabilisers.
Packaging: Packed in plastic bottle.
Formula:
C₁₆H₂₀N₂ MW: 240.35 g/mol |
MDL Number:
MFCD00007748 CAS Number: 54827-17-7 |
Specification Test Results
Abs.@450nm (50%, 1M H2SO4) | ≤ 0.1 |
Abs.@650nm (undiluted) | ≤ 0.1 |
Assay (P/F) | PASS / FAIL |
pH @25C (undiluted) | 2.6 - 3.6 |
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