Proteinase K Solutions, 20 mg/ml

Supplier: EMPIRICAL BIOSCIENCE

PKS-10ML PKS-5ML

77676-332EA 200.67 USD

77676-332 77676-334

Proteinase K Solutions, 20 mg/ml

Enzymes

Proteinase K is a broad-spectrum endopeptidase derived from the fungus Tritirachium album. The native enzyme has a size of 26 to 28 kDa and preferentially cleaves peptide bonds adjacent to aliphatic and aromatic amino acid residues of both native and denatured proteins.

Applications: Sample processing

Proteinase K can be used to enhance cell and viral particle lysis in combination with a variety of detergents (Tween 20, Triton X-100, SDS, Sarkosyl) and denaturants (Guanidinium, urea) during nucleic acid extraction.

Empirical's Proteinase K is stable over a wide range of pH values (pH 4 to 12) and temperatures (30 to 60 °C) with optimal activity at pH 8.0 and 55 °C. Enzyme activity is maintained in the presence of reductants such as 2-mercaptoethanol and DTT (≤5 mM), as well as the metal ion chelator EDTA (≤50 mM).

Note that Proteinase K is sensitive to common serine protease inhibitors including PMSF, AEBSF and DFP, which should be left out of lysis buffers used for nucleic acid extraction.

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