RNAspin Mini Kits, Cytiva

Supplier: Cytiva
25-0500-71 25-0500-70 25-0500-72 25-0500-87 25-0500-87 25-0500-70 29489363 25-0500-71 25-0500-72
95017-493PK 1734.95 USD
CA95017-489L CA95017-493L 95017-493 95017-491 10035-986 MPCA29489363 CA95017-491L CA10035-986 95017-489
RNAspin Mini Kits, Cytiva
Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents
RNAspin Mini RNA Isolation Kit is a complete RNA purification kit designed for rapid extraction of high-quality total RNA from a wide range of sample types.

  • High quality output RNA from diverse sample types
  • Recovers high quality total RNA due to the removal of genomic DNA through on-column DNase I treatment
  • For maximum yield and purity, prefilters are included to reduce lysate viscosity
  • Results can be obtained with even small amounts of precious sample (e.g. >10 HeLa cells for RT-PCR)
  • Lysis buffer less susceptible to foaming to ensure valuable RNA sample is not wasted
  • Simple and convenient format that is suitable for all levels of expertise

RNA extraction is the purification of RNA from biological samples. It is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA, since it is less stable than DNA.

Besides other methods in the RNA isolation protocol to help counter RNA degradation, the equipment used for RNA preparation is usually kept separate from other lab equipment and treated (or has been pre-treated) with chemicals that can destroy RNases, such as diethyl pyrocarbonate (DEPC). RNAspin Mini Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria, or yeast. The isolated RNA is of a high enough quality and quantity (up to 100 μg) for multiple downstream experiments and sensitive enzymatic applications like RT-PCR, real time PCR, primer extension, RNAseq, and RNase protection assays.
The RNAspin Mini Kit has been used for the isolation of high-quality total RNA from several types of source material including hard-to-lyse bacterial strains and can be applied in downstream experiments such as quantitative reverse transcriptase PCR (QRT-PCR), Northern blot, and microarray experiments. The kit is supplied with a full RNA extraction protocol booklet with a detachable, quick reference protocol card, and all the necessary components including prefilters and DNase I.

Delivery information: The kit is supplied with a full protocol booklet with a detachable, quick reference protocol card, and all the necessary components including prefilters and DNase I.
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