The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.
- Microcentrifuge spin cup method is scaled and optimised for treating 10 µl human serum samples for 2D electrophoresis
- Removes nearly all HSA and IgG, enabling low-abundance proteins to be detected in 2D gels
- Kit processes samples in less than 40 minutes
- Scalable - can use different amounts of affinity resin to match sample volume and concentration
These antibody-based kits make use of specific antibodies against human serum albumin (HSA) and human gamma immunoglobulin (IgG), which are immobilized to beaded agarose gel
The antibody-based kit provides the most specific method for removing HSA and IgG. The standard removal kit uses a mixture of immobilized Cibacron® blue and immobilized protein A gel to quickly and easily remove albumin and IgG. This kit may be used to treat samples from monkeys, swine, rabbits.
When samples are incubated with the antibody gel, both HSA and IgG bind. Various size samples up to 600µg are easily purified. Simply use different amounts of gel with the included spin columns.