The Thermo Scientific Pierce Ubiquitin Enrichment Kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.
- Fast—less than 45 minutes hands-on time for binding and recovery of ubiquitinated proteins
- Complete—includes all reagents needed for ubiquitin-modified protein enrichment from cultured cells and tissue samples
- Flexible—sample incubation from 2 hours to overnight allows assay to be completed in several hours or in less than 30 minutes after overnight incubation
- Robust—compatible with all Thermo Scientific Pierce Cell Lysis Products and standard RIPA formulations
- Multiple-sample format—easily processes 1 to 15 samples concurrently
The enriched fraction can subsequently be analyzed be quantitatively or qualitatively, depending on the application. The spin-column protocol requires less than 45 minutes of hands-on prep time and incubation can range from two hours to overnight for flexibility in scheduling assay times. The components in the kit are compatible with all Poppers cell lysis products and standard RIPA formulations. It will process up to 15 samples concurrently.
Delivery information: The kit contains Polyubiquitin Positive control (1000X, 50 µL), Anti-Ubiquitin Antibody (Rabbit antiserum, 5 µL), Polyubiquitin affinity resin slurry (300 µL), TBS pack (makes 500 mL), and Spin Columns (18) and Accessories.