X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) solution, Novagen®, Sigma-Aldrich®

Supplier: MilliporeSigma

Synonyms: BCIGX-GAL(2S,3R,4S,5R,6R)-2-((5-Bromo-4-chloro-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol

M-Clarity™ quality level = MQ100

71077-3
EM71077-3EA 185.49 USD
EM71077-3
X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) solution, Novagen®, Sigma-Aldrich®
X-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

Convenience for blue/white screening.


X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing.[1] X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.

Formula: C₁₄H₁₅BrClNO₆
MW: 408.63 g/mol
Storage Temperature: Freezer
MDL Number: MFCD00005666
CAS Number: 7240-90-6

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