X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) solution, Novagen®, Sigma-Aldrich®
Supplier: MilliporeSigma
Synonyms:
BCIG
, X-GAL
, (2S,3R,4S,5R,6R)-2-((5-Bromo-4-chloro-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
M-Clarity™ quality level = MQ100
Convenience for blue/white screening.
X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing.[1] X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.
Formula:
C₁₄H₁₅BrClNO₆ MW: 408.63 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00005666 CAS Number: 7240-90-6 |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...