rPSL1a-LecBeads [for Siaα(2-6)Gal]

Supplier: TCI

R0235-1VIAL

TCR0235-1VIALEA 567.53 USD

TCR0235-1VIAL

rPSL1a-LecBeads [for Siaα(2-6)Gal]

Protein Purification Protein Purification Resins

The rPSL1a is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Polyporus squamosus. This lectin preferably binds to a [Siaα(2-6)Gal] glycan epitope (It very slightly interacts with lactose.).
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rPSL1a-LecBeads (1 ml gel) shows a binding capacity of about 10 mg or more human Transferrin [hTF] through its binding to a terminal [Siaα(2-6)Gal] glycan epitope on hTF.

Preparation : Recombinant PSL1a is covalently coupled with agarose gel
Quantity : 1 ml gel/2 ml (1:1, v/v slurry) suspended in PBS, pH7.5
Protein concentration : about 10 mg/ml gel
Binding capacity : >10 mg of glycoprotein (human Transferrin) /ml gel
Preservative : 0.02% [w/v] Sodium azide
Stabilizer : None
Store at 2-10 °C. (Avoid freezing).

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