A fast homogeneous, fluorometric method for estimating the number of non-viable cells present in multiwell plates. Based on detection of LDH release, an indicator of cytotoxicity.
- A Fluorometric Method for Estimating the Number of Nonviable Cells Present in Multiwell Plates
- Elimination of cell transfer step saves time
- Perform multiple assays on one sample
The CytoTox-ONE Homogeneous Membrane Integrity Assay is a fluorometric method for estimating the number of nonviable cells present in multiwell plates. The CytoTox-ONE Assay rapidly measures the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the culture medium is measured with a 10-minute coupled enzymatic assay that results in the conversion of resazurin into a fluorescent resorufin product. The amount of fluorescence produced is proportional to the number of lysed cells using a 96- or 384-well format. The CytoTox-ONE Reagent does not damage normal healthy cells; therefore the reactions to measure released LDH can be performed directly in a homogeneous format in assay wells containing a mixed population of viable and damaged cells. The CytoTox-ONE Homogeneous Membrane Integrity Assay, HTP (Cat.# G7892), offers convenient, alternative packaging for processing multiple plates. Each bottle of reagent supplied with the system is sufficient to perform 500 assays in a 96-well format or 2,000 assays in a 384-well format when the recommended volumes are used.
CytoTox-ONE™ is a fluorometric method for determining the number of nonviable cells present in multiwell plates. This system rapidly measures lactate dehydrogenase (LDH) released through damaged cell membranes into the culture medium. CytoTox-Fluor™ cytotoxicity assay measures the relative number of dead cells in a population in as little as 30 minutes. The assay is based on measurement of a conserved and constitutive protease activity released from membrane-compromised cells. It can also be used in single-well, sequential, multiplex format with other downstream assay chemistries to normalize data for cell number or to obtain more relevant information per well.
The assay is based on measurement of conserved and constitutive protease activity released from membrane-comprised cells. It provides a linear response and can distinguish between small differences in viability across the entire cytotoxicity spectrum, providing superior sensitivity over other methods. The assay can also be used in single-well, sequential, and multiplex format with other downstream assay chemistries to normalize data for cell number or to obtain more relevant information per well.
