Radiant™ HiFi Polymerase
Supplier: Alkali Scientific
Achieve high-fidelity amplification with Radiant™ HiFi polymerase, ensuring accurate and reliable PCR results.
- >50-fold higher fidelity than standard Taq DNA polymerase
- New-generation PCR buffer formulation for maximum PCR efficiency and reaction speed
- Robust PCR performance across a wide range of DNA templates including genomic DNA and GC-rich and AT-rich sequences
- Supplied with 5X HiFi Ultra Reaction Buffer containing MgCl2 and highly pure dNTPS
- High DNA yields with either fast cycling or standard cycling
Ultra-High Fidelity Including dNTP's Radiant™ HiFi Ultra DNA Polymerase is a high-performance, high-fidelity DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA. Radiant™ HiFi Ultra Polymerase possesses 5´to 3´ DNA polymerase and 3´to 5´ proofreading exonuclease activities with an error-rate of 4.55×10⁻⁷. The polymerase is ideal for cloning applications, library construction, screening and genotyping of problematic GC-rich and AT-rich sequences. Radiant™ HiFi Ultra Polymerase is engineered for robust, superior high-fidelity PCR in comparison with standard proof-reading enzymes such as pfu DNA Polymerase. Several point-mutations combined with a new-generation buffer formulation provide for significantly improved processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples. The supplied 5X HiFi Ultra Reaction Buffer contains an ideal concentration of MgCl₂ and highly pure dNTPS for convenience and consistency in liquid handling. Storage Radiant™ HiFi Ultra DNA Polymerase is shipped on blue or dry ice and should be stored at –20 °C upon receipt. Excessive freeze/thawing should be avoided. When stored as specified, Radiant™ HiFi Ultra DNA Polymerase is stable for 12 months from date of receipt. The kit may also be stored at 4 °C for 1 month. Important Considerations Radiant™ 5X HiFi Ultra Reaction Buffer: The 5x reaction buffer contains 15 mM MgCl₂, 5 mM dNTPs, and PCR enhancers. The buffer composition has been optimized for maximum efficiency, sensitivity and success with difficult amplicons. We do not suggest the use of additional MgCl₂ or enhancers. Template: For complex genomic DNA, we suggest the use of 5 to 500 ng per reaction.
For cDNA or plasmid DNA, please use <100 ng per reaction. Primers: Primers should have a predicted melting temperature of around 60 °C, using default Primer 3 settings. The final primer concentration in the reaction should be between 0.2 and 0.6 μM. Annealing: We recommend performing a temperature gradient to determine the optimal annealing temperature. Alternatively, we suggest a 57 °C annealing temperature and then an increase in 2 °C increments if non-specific products are present. Extension: Optimal extension is achieved at 72 °C. The optimal extension time is dependent on amplicon length and complexity. 30 seconds per kilobase (Kb) is recommended for amplification from eukaryotic genomic DNA or cDNA.
Quality control Radiant™ HiFi Ultra DNA polymerase is tested extensively for robust activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination. Radiant™ HiFi ultra DNA polymerase is manufactured under a comprehensive quality management system, following ISO 9001:2008 standards.
Caution: This product is intended for research purposes only and is not intended for any animal or human therapeutic use.
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