Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
Supplier: RPI
TRIS, also known as tris(hydroxymethyl)aminomethane, is a chemical compound widely used in life science as a buffer. TRIS is commonly employed in biological and biochemical research to maintain a stable pH in various experimental procedures. It is valued for its buffering capacity and its ability to maintain a constant pH in a desired range.
It is commonly used in applications such as protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques. TRIS is highly effective in buffering solutions in the pH range of 7.0 to 9.2, making it suitable for a wide range of biological processes and experimental conditions. It is particularly useful for maintaining a stable pH in the alkaline range. One of the significant advantages of TRIS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
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Formula:
NH₂C(CH₂OH)₃ Melting Pt: 171...172 °C Storage Temperature: Room Temperature |
MDL Number:
MFCD00004679 CAS Number: 77-86-1 EINECS: 201-064-4 |
Specification Test Results
| Appearance | White Crystalline Powder |
| Absorbance at 280 nm (1M Solution) | ≤0.05 |
| Absorbance at 300 nm (25% Solution) | ≤0.04 |
| APHA Color, 20% Aqueous solution | ≤25 |
| Arsenic (As) | ≤5 ppm |
| Assay (Titration, dried basis) | 99.5 - 101.0% |
| Chloride | ≤1 ppm |
| Copper (Cu) | ≤1 ppm |
| DNase, RNase, protease | Not Detected |
| Electrophoresis | Pass Test |
| Heavy Metals (as Pb) | ≤1 ppm |
| Identification (IR) | Pass |
| Iron (Fe) | ≤1 ppm |
| Magnesium (Mg) | ≤10 ppm |
| Melting Point | 168.0 - 172.0 °C |
| Moisture (KF) | ≤0.5% |
| pH (5% Solution) | 10.0 - 11.5 |
| Residue on Ignition | ≤0.1% |
| Solubility (40% Aqueous Solution) | Clear |
| Sulfate | ≤5 ppm |
| Turbidity (40% Solution) | ≤2.0 |
| UV Absorbance at 260 nm, 10% Aqueous solution | ≤0.1 |
| UV Absorbance 280 nm (10% Solution in water) | ≤0.08 |
| UV Absorbance at 430 nm, 10% Aqueous solution | ≤0.009 |
| BSE/TSE Free | BSE/TSE Free |
| Non Animal Origin | Non Animal Origin |
| Penicillin or other Beta Lactams | None Present |
| Country of Origin | Report |
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