NEXTFLEX™ Poly(A) Beads 2.0

Supplier: Revvity Health Sciences
NEXTFLEX™
NOVA-512994 NOVA-512995 NOVA-512991 NOVA-512993 NOVA-512995 NOVA-512991 NOVA-512994 NOVA-512993 NOVA-512992 NOVA-512992
77448-146EA 407.34 USD
77448-146 CA77448-144 77448-144 CA77448-142 77448-142 CA77448-140 77448-140 CA77448-138 77448-138 CA77448-146
NEXTFLEX™ Poly(A) Beads 2.0
Nucleic Acid Reagents Next Generation Sequencing Reagents
NEXTFLEX™ poly(A) beads 2.0 kits offer a convenient method for obtaining pure, intact mRNA upstream of RNA-sequence library prep with improved mRNA yields, low rRNA contamination and a simple protocol.

  • 10 ng - 5 µg total starting RNA
  • Magnetic bead-based protocol
  • No organic solvents or precipitation step required
  • Optimized for use with 10 ng - 5 µg of total RNA as starting material
  • Versions are available for automated and manual isolation of the mRNA transcriptome
  • Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations

Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples for RNA-sequence library preparation. Removal of rRNA avoids the waste of reagents and bioinformatics resources to sequence and align ~85% of total RNA comprising rRNA (which is generally not a target of interest in NGS sequencing experiments). Revvity’s NEXTFLEX™ poly(A) beads 2.0 kit offers an easy and cost-effective method for removing rRNA in RNA-sequence libraries. This product takes advantage of the tract of adenosine residues present at the 3’ ends of a vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines ('oligo dT') immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.

Certifications: Manufactured in ISO 9001 facility.

Caution: For research use only. Not for use in diagnostic procedures.
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