NEXTFLEX™ RiboNaut rRNA (HMR) Depletion Kits

Supplier: Revvity Health Sciences
NEXTFLEX™
NOVA-512965 NOVA-512961 NOVA-512964 NOVA-512965 NOVA-512964 NOVA-512962 NOVA-512963 NOVA-512961 NOVA-512963 NOVA-512962
77448-136EA 5242.7 USD
CA77448-134 77448-136 CA77448-132 77448-134 CA77448-130 77448-132 77448-130 CA77448-128 77448-128 CA77448-136
NEXTFLEX™ RiboNaut rRNA (HMR) Depletion Kits
Nucleic Acid Reagents Next Generation Sequencing Reagents
The NEXTFLEX® RiboNaut™ rRNA depletion kit (HMR) effectively removes rRNAs from mammalian total RNA while enabling labs to find additional RNA species in a sample, not only limited to intact mRNAs.

  • Hybridization and bead-based protocol for rRNA depletion
  • Optimized for use with 5 ng to 1 µg of total RNA as starting material
  • Versions are available for automated and manual rRNA depletion

The NEXTFLEX® RiboNaut™ rRNA depletion kit (Human/mouse/rat) is designed to be compatible with human, mouse, and rat total RNA. The method used in the NEXTFLEX® RiboNaut™ rRNA depletion kit is based on subtractive hybridization, using a mixture of biotinylated oligonucleotides complementary to rRNAs.

After hybridization of the bait oligos to total RNA, the rRNA/bait complex and excess bait oligos are removed by capture onto streptavidin-coated magnetic beads. The remaining RNA, which has been depleted of both cytoplasmic and mitochondrial rRNAs, is then purified and concentrated using magnetic beads.

Certifications: Manufactured in ISO 9001 Facility.

Caution: For research use only. Not for use in diagnostic procedures.
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