IPTG (isopropyl-β-D-thiogalactopyranoside) ≥99%, white powder
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Warning
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Synonyms:
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol
, IPTG
, Isopropyl Beta-D-1-thiogalactopyranoside
IPTG (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli.
IPTG is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal in blue-white color selection of recombinant bacterial colonies.
The final concentration typically used in indicator plates is 0.2 mM.
- Presentation: Crystalline, Dioxane-free,β-Galactosidase inducer
- Soluble in methanol and water (50 mg/mL - clear, colorless solution)
- Storage temperature: -20°C, desiccate
Formula:
C₉H₁₈O₅S MW: 238.31 g/mol Boiling Pt: 402 °C (1013 hPa) Melting Pt: 105…115 °C Storage Temperature: Freezer |
MDL Number:
MFCD00063273 CAS Number: 367-93-1 EINECS: 206-703-0 |
Specification Test Results
Identity Test | Passes |
Purity | ≥99% |
FTIR | Conforms to Standard |
pH (5% aq) | 5.0-7.0 |
Optical Rotation (c=1, water) | -26° to -33° |
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