HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥99%, Ultrapure Bioreagent for liquid chromatography, for molecular biology, J.T.Baker®
Supplier: AVANTOR PERFORMANCE MATERIAL LLC
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Warning
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Synonyms:
4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid
, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid
, N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid
, 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid
, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)
, N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid
, HEPES free acid
Management of Change (MOC) category = N
Free acid for liquid chromatography and molecular biology buffers. Lot analysis on label.
- Lot analysis on label
Formula:
C₈H₁₈N₂O₄S MW: 238.31 g/mol Melting Pt: 210…215 °C Density: 1.448 g/cm³ (20 °C) Storage Temperature: Ambient |
MDL Number:
MFCD00006158 CAS Number: 7365-45-9 EINECS: 230-907-9 Merck Index: 13,04672 |
Specification Test Results
For Laboratory,Research,or Manufacturing Use | |
Assay (C₈H₁₈N₂O₄S) | ≥99 % |
Appearance (fine, white, crystalline powder) | Passes Test |
Sodium (USP Id.) | Passes Test |
DNase Activity (None Detected) | Passes Test |
RNase Activity | Passes Test |
Protease Activity | Passes Test |
Absorbance of a 0.1M Solution (1-cm path vs water) at - 260 nm | ≤0.050 |
Absorbance of a 0.1M Solution (1-cm path vs water) at - 280 nm | ≤0.050 |
pH of 5% Solution at 25°C | 5.0 - 6.5 |
Ash (sulfated) | ≤0.2 % |
Arsenic and Antimony (as As) | ≤0.050 ppm |
Trace Impurities - ACS - Heavy Metals (as Pb) | ≤5 ppm |
Trace Impurities - Iron (Fe) | ≤5 ppm |
pK^a at 20°C, For Information Only | |
QC Supervisor or equivalent to check supplier's COA to ensure proper chemical form. | Passes Test |
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