For quantitative determination of ß-glucuronidase enzyme activity in tissue and bacterial samples.
- Highly sensitive
- The fluorimetric nature of the kit detects 1×10⁻⁴ to 8 U/L BG activity in 96 well plate with only 10 μl sample within 30 min
- Versatile
- This assay has been validated to work with both animal and E.coli derived β-glucuronidase
- High-throughput
- Homogeneous 'mix-incubate-measure' type assay
- Can be readily automated to assay thousands of samples per day
BETA-GLUCURONIDASE (BG) is an enzyme which catalyzes the hydrolysis of β-glucuronide bonds. In humans, β-glucuronidase catalyzes the hydrolysis of β-D-glucuronic acids from glycoproteins and mucopolysaccharides such as heparan sulfate. β-glucuronidase activity is important for drug metabolism as drugs are often conjugated to glucuronic acid to make for a more water soluble delivery system, relying on subsequent β-glucuronidase activity to release the active drug. Bilirubin clearance is also facilitated by glucuronidation. GUSB is often used as a reporter gene to track gene expression through β-glucuronidase activity. BioAssay Systems’ fluorimetric BG assay uses a β-D-glucuronide substrate which fluoresces upon hydrolysis with β-glucuronidase and is read at 365/450 nm.
