Diethyl pyrocarbonate, clear, colorless liquid
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Warning
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Diethyl Pyrocarbonate (DEPC) is a chemical used to inactivate RNase enzymes and is sensitive to moisture and pH. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.
DEPC is effective as a nuclease inhibitor. Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. Diethyl Pyrocarbonate is used to modify proteins and nucleic acids.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation. It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles.
- Presentation: Clear, Colorless Liquid
- Refractive Index: n20/D 1.398(lit.)
- Soluble in alcohols, esters, ketones, hydrocarbons. A 50% w/w solution in ethanol may be prepared. Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2.
- Storage temperature: Store at +4 °C, store under nitrogen
DEPC is effective as a nuclease inhibitor.Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
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Formula:
O(COOC₂H₅)₂ Boiling Pt: 93…94 °C (18 mmHg)) (lit.) Density: 1.101 g/mL (25 °C) (lit.) Flash Pt: 69 °C Storage Temperature: Refrigerator |
MDL Number:
MFCD00009106 CAS Number: 1609-47-8 EINECS: 216-542-8 Merck Index: 13,08088 |
Specification Test Results
| Identity Test | Passes |
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