Dithiothreitol (DTT, Cleland's reagent) ≥99.0%, white powder
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Warning
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Synonyms:
DL-1,4-Dithiothreitol
, DTT
, cis-2,3-Dihydroxy-1,4-butanedithiol
, (R*,R*)-1,4-Dimercapto-2,3-butandiol
DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
DTT has been used:
- as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk.
- as a component of medium for the demembranation and reactivation of spermatozoa.
- to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.
- as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Soluble in water (50 mg/mL - clear and colorless solution); soluble in ethanol, acetone, ethylate, chloroform and ether.
Caution: Research Use Only (RUO).
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Formula:
C₄H₁₀O₂S₂ MW: 154.25 g/mol Boiling Pt: ∼125 °C (1013 hPa) Melting Pt: 40…50 °C (lit.) Flash Pt: >110 °C Storage Temperature: Refrigerator |
MDL Number:
MFCD00004877 CAS Number: 3483-12-3 EINECS: 222-468-7 UN: 3335 ADR: 9,III |
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Specification Test Results
| Identity Test | Passes |
| Purity (Titration) | ≥99.0% |
| Melting Point | 40 - 50 °C |
| FTIR | Conforms to Standard |
| OD Readings | For Information Only |
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