3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulfate (CHAPS) ≥98%, white powder
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Warning
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CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents.
- pH: 4.0 - 7.0 (10% aq soln)
- Aggregation Number: 4-14 (0-0.1 M Na+)
- Critical Micelle Concentration (CMC): 6-10 mM
CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.
CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 1-4% (v/v) are typically used in an isoelectric focusing gel. A commonly used isoelectric focusing sample solution consists of 8 M urea, 4% CHAPS, 50-100 mM dithiothreitol (DTT) and 40 mM Tris.4 Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.
Soluble in water (100 mg/ml - clear, colorless to slightly yellow solution).
Caution: Research Use Only (RUO).
Formula:
C₃₂H₅₈N₂O₇S Melting Pt: 265 °C (lit.) Storage Temperature: Ambient |
MDL Number:
MFCD00081083 CAS Number: 75621-03-3 Merck Index: 13,02052 |
Specification Test Results
Identity Test | Passes |
Purity | ≥98% |
FTIR | Conforms to Standard |
OD400 (5% aq soln) | Report Result |
OD290 (5% aq soln) | Report Result |
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