Agarose, white powder for electrophoresis
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae.
Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions. Agarose was used to prepare 0.5% agarose gel for Southern blot analysis of the DNA extracted from primate muscles.
The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
- Electrophoresis Grade
- DNase, RNase: None Detected
- Soluble in water
- Room Temperature
Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae with an Electroendoosmosis(EEO) value of 0.13 making it an essential component in chemical separation techniques. It has a gel strength of ≥600 gm/cm² and Gel Temperature:36°C.It is specially prepared to allow recovery of active DNA after separation, with minimal inhibition of restriction and ligation enzymes. Suitable for both analytical and preparative electrophoresis procedures.This is a standard agarose with good gel strength and clarity, suitable for all general applications.
Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.Agarose was used to prepare 0.5% agarose gel for Southern blot analysis of the DNA extracted from primate muscles (Magalie Penaud-Budloo ,Adeno-Associated Virus Vector Genomes Persist as Episomal Chromatin in Primate Muscle ,2008,Journal of Virology, 82(16),7875-7885 ).
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Melting Pt:
87.3…89.5 °C Storage Temperature: Ambient |
MDL Number:
MFCD00081294 CAS Number: 9012-36-6 EINECS: 232-731-8 |
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Specification Test Results
| Identity Test | Passes |
| Electroendosmosis | 0.11-0.15 |
| Gel Strength (1.5% gel) | ≥600 g/cm2 |
| Moisture | ≤5% |
| pH (1% aq soln) | 6-7.5 |
| Sulfur | ≤0.15% |
| Gel Temperature | 35-37°C |
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