DIBMA glycerol in HEPES, PureCube for protein solubilisation
Supplier: CUBE BIOTECH
With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017. One challenge when working with traditional DIBMA is its sensitivity to the presence of ions inside the buffer/ media. The reason for this is DIBMA's own charge that lets it interact with these ions. This leads to the precipitation of DIBMA and therefore loss of its function. To overcome this issue, we modified DIBMA to have a reduced charge to reduce the affinity to the ions drastically. This was achieved by adding Glucosamine or an amino-functionalized diol to DIBMA. This modified DIBMA can be used for experiments for which the presence of ions is crucial for their success.
Usage: Protein solubilization
Formula Weight 10,000 g/mol
pH:7.5 in buffer
dn/dc: 1.35 M-1
Solubility > 10 % in H2O
Absorbance at 280 nm: < 0.3 (1 % solution)
Mg2+ Tolerance: >50 mM
Ca2+ Tolerance: >50 mM
Caution: Shipping Temperature: Ambient temperature. Storage of lyophilized copolymer: -20°C for several years. Storage of dissolved copolymer: 2-8°C for several day.
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