DIBMA glucosamine in HEPES, PureCube for protein solubilisation
Supplier: CUBE BIOTECH
With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.
Usage: Protein solubilization
Formula Weight 10,000 g/mol
pH: 7.5 in buffer
dn/dc: 1.35 M-1
Solubility: > 10 % in H2O
Absorbance at 280 nm: < 0.3 (1 % solution)
Mg2+ Tolerance: >50 mM
Ca2+ Tolerance: >50 mM
Caution: Shipping Temperature: Ambient temperature. Storage of lyophilized copolymer: -20°C for several years. Storage of dissolved copolymer: 2-8°C for several day.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...