PureCube Glutathione Agarose XL

Supplier: Cube Biotech
PureCube
57010 57003 57005 57012
77393-318EA 9070.18 USD
77393-318 77393-316 77393-314 77393-312
PureCube Glutathione Agarose XL
Protein Purification Protein Purification Reagents
The protein purification with the PureCube Glutathione Agarose and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.

  • Usage: Specific binding and purification of GST-tagged proteins
  • Specificity: Affinity to GST(Glutathione-S-Transferase)-tagged proteins
  • Binding capacity: >2 mg/ml
  • Bead ligand: L-Glutathione (reduced)
  • Bead size: 400 μm
  • Matrix: 7.5% cross-linked agarose
  • Filling quantity: Delivered as a 50% suspension
  • pH stability: 3 to 12

The pairing of an enzyme to its substrate is one of the most specific combinations in life sciences. This is why GST-tag purifications exploit this specificity to gain high purity levels while maintaining a high protein yield. PureCube Glutathione Agarose XL is a suspension of 400 µm purification beads used for this purpose, although specialized for high flow rates and viscous cell media. They are applicable to all sorts of affinity chromatography methods, such as FPLC, batch spin, or column chromatography.

Required equipment:
-Lysis buffer
-Wash buffer
-Elution buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 10000×g)
-50 ml centrifuge tube
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents and equipment; Optional: Western Blot reagents and equipment
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