Nanodisc Mouse MSP1D1-His DMPC

Supplier: Cube Biotech
26711
77392-794EA 768 USD
77392-794
Nanodisc Mouse MSP1D1-His DMPC
Protein Expression Protein Expression Reagents
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

  • Origin of MSP protein: Mouse
  • Purity: >90% (Determined by SDS-PAGE)
  • Number of amino acids: 214
  • Molecular mass: 24928 Da
  • Extinction coefficient (in water) ε280: 22460 M⁻¹cm⁻¹
  • Buffer: 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA
  • Affinity tag: N-Terminal His-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 mouse creates nanodiscs of ∼9 to 10 nm in diameter. Note that the MSP proteins in these nanodiscs are His-tagged. Keep that in mind when tagging your membrane protein of interest.

There are multiple phospholipids to choose from that can make up the body of the nanodisc. Which is best, depends on your protein.
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