DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations.
DIBMA 10:
Usage: Protein solubilization
Formula weight 10000 g/mol
pH: 7.5 in buffer
dn/dc: 1.35 M⁻¹
Solubility >10% in H₂O
Absorbance at 280 nm: <0.3 (1% solution)
Mg²⁺ Tolerance: 25 mM
Ca²⁺ Tolerance: starts to precipitate at 50 mM
Shipping temperature: Ambient temperature
Storage of lyophilized copolymer: –20 °C for several years
Storage of dissolved copolymer: 2 to 8 °C for several day
DIBMA 12:
Formula weight 12000 g/mol
pH: 7.5 in buffer
dn/dc: 1.35 M⁻¹
Solubility >10% in H₂O
Absorbance at 280 nm: <0.3 (1% solution)
Mg²⁺ Tolerance: 25 mM
Ca²⁺ Tolerance: starts to precipitate at 50 mM
DIBMA GLYCEROL:
Usage: Protein solubilization
Formula weight 10000 g/mol
pH: 7.5 in buffer
dn/dc: 1.35 M⁻¹
Solubility >10% in H₂O
Absorbance at 280 nm: <0.3 (1% solution)
Mg²⁺ Tolerance: >50 mM
Ca²⁺ Tolerance: >50 mM
DIBMA GLUCOSAMINE:
Usage: Protein solubilization
Formula weight 10000 g/mol
pH: 7.5 in buffer
dn/dc: 1.35 M⁻¹
Solubility >10% in H₂O
Absorbance at 280 nm: <0.3 (1% solution)
Mg²⁺ Tolerance: >50 mM
Ca²⁺ Tolerance: >50 mM
Delivery information: Shipping temperature: Ambient temperature; Storage of lyophilized copolymer: −20 °C for several years; Storage of dissolved copolymer: 2 to 8 °C for several day.
