This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
- Origin of protein: Human
- Purity: >90% (Determined by SDS-PAGE)
- Number of amino acids: >217
- Molecular mass: 25309 Da
- Extinction coefficient (in water) ε280: 25440 M⁻¹ cm⁻¹
- Buffer: 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA
- Sequence (1-Letter Code): MGSSHHHHHH ENLYFQ GLKLLDNWDSVTSTFSKLREQLG PVTQEFWDNLE KETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFK VSFLSALEEY TKKLNTQ
- Affinity tag: N-Terminal His-tag
Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 creates nanodiscs of ∼9 to 10 nm in diameter. This product contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein.
Delivery information: Shipping temperature: Dry-Ice; storage of reconstituted protein: −80 °C, avoid freeze-thaw cycles.
