Nanodisc Assembly Kit MSP1E3D1-His POPC

Supplier: Cube Biotech
26253
77392-704PK 407.88 USD
77392-704
Nanodisc Assembly Kit MSP1E3D1-His POPC
Protein Expression Protein Expression Reagents
This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1E3D1 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

  • Origin of protein: Human
  • Purity: >90% (Determined by SDS-PAGE)
  • Number of amino acids: >217
  • Molecular mass: 25309 Da
  • Extinction coefficient (in water) ε280: 25440 M⁻¹cm⁻¹
  • Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
  • Sequence (1-Letter Code): MGSSHHHHHH ENLYFQ GLKLLDNWDSVTSTFSKLREQLG PVTQEFWDNLE KETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFK VSFLSALEEY TKKLNTQ
  • Affinity tag: N-Terminal His-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences to stabilize membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1E3D1 (His-tagged) creates nanodiscs of ∼12 to 14 nm in diameter.

With this assembly kit both cellular as well as cell-free expressed proteins can be stabilized in MSP nanodiscs. Just use the corresponding protocol for each purpose (see below). The MSP protein contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein.

Delivery information: Shipping temperature: Room temperature; storage of lyophilized copolymer: −20 °C for several years; storage of reconstituted protein: 2 to 8 °C for several day.
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