Authenticase™ Nucleases

Supplier: New England Biolabs (NEB)

Authenticase™ Nucleases

Authenticase™ Nucleases

M0689L M0689S

77322-318EA 600 USD

77322-318 77322-320

Authenticase™ Nucleases

Enzymes

Authenticase™ is a proprietary mixture of structure-specific nucleases capable of recognizing and cleaving outside mismatch and indel (insertion and/or deletion) regions, ranging from 1 to 10 basepairs (bp) on double-stranded DNA.

Cleave heteroduplex DNA
Improve accuracy in gene synthesis
Supplied with 5X DNA anneal buffer and 10X reaction buffer

Authenticase is a mixture of enzymes capable of recognizing mismatch and indel regions on a double-stranded DNA and creating double-stranded cleavages around these regions and with a limited non specific activity on homoduplex regions of DNA. The regions of mismatch or indel could range from at least 1 to 10 bps. The activity of cleavage on mismatch/Indel regions can be utilized in the process of oligo-based PCR gene synthesis to improve the accuracy of PCR amplicons. Alternatively, Authenticase™ treated products can be analyzed to determine the popularity of heteroduplex of a PCR pool amplified from enzyme-based edited genome.

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