NEBNext® UltraShear™ DNA Library Preparation Kits

Supplier: New England Biolabs (NEB)

M7634L M7634S

77025-078EA 449 USD

77025-078 77025-088

NEBNext® UltraShear™ DNA Library Preparation Kits

Nucleic Acid Reagents Next Generation Sequencing Reagents

NEBNext UltraShear fragments DNA in a time-dependent manner and the same protocol is followed for all input amounts (5 to 250 ng).

Does not affect methylation marks
Methylation analysis: improves CpG coverage and sequencing metrics
FFPE DNA: improves usable reads and decreases artificial mutation frequency
Single protocol for all input amounts
Increased library yields

Enzymatic fragmentation of DNA as part of the library prep workflow provides many advantages compared to mechanical shearing. However, specialized fragmentation reagents are required for enzymatic shearing of challenging samples such as FFPE DNA, and in order to maintain methylation marks on samples for methylome analysis. NEBNext UltraShear is a mix of enzymes that has been designed and optimized to fragment these sample types upstream of library preparation. This improves library yields and diversity, and allows retention of methylation marks.

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