Red Taq DNA Polymerase is a blend of VWR® Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.
- Ideal choice for routine applications
- High performance, thermostable DNA polymerase
- Optimal for TA cloning
- dUTP incorporation possible
- Processes up to 5 kb
- Leaves a 3'dA overhang
Red Taq DNA polymerase concentration: 5 Units/μl
10X Key Buffer: Tris-HCl pH 8.5; (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween® 20
10X Extra Buffer: Tris-HCl pH 8.3; KCl, 15 mM MgCl₂, 1% Tween®20
Taq DNA polymerase is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. The enzyme lacks a 3'→5' exonuclease activity (no proofreading ability). It leaves an A' overhang, which makes the enzyme ideal for TA cloning.
Taq DNA polymerase is suitable for standard testing, routine PCR, screening and high throughput testing.
Long term storage at -20 °C. Product expiry at -20 °C is stated on the label. Optional: Store at +4 °C for up to 6 months.
Red Taq DNA Polymerase is supplied with both Key Buffer and Extra Buffer. Additional MgCl₂ is included with each kit to enable optimization.
Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) minimizing the need for optimization of the Mg²⁺ concentration or the annealing temperature in most primer-template systems. Extra Buffer is a traditional potassium (K⁺) buffer. Extra Buffer promotes high specificity, but careful optimization of primer annealing temperatures and Mg²⁺ concentrations may be required.
Caution: For Research Use Only. Not for use in diagnostic procedures.
