ADAM™ CellT is an automated cell counter that complies with the 21 CFR Part 11 which is a regulation on electronic records and signatures for use in cGMP facilities. Data cannot be modified by any user. Every action of users is recorded in an audit trail which includes the date, time, and specific details of the action.
- Reports number of total cells, viable cells, non-viable cells and cell viability (%)
- Cell type: Cell line (clumpy cell, single cell), PBMCs, adipose stem cell, total white blood cell, primary cells
- Ideal for debris containing clumped or irregular shaped cell populations
- Short counting time of only 25 seconds
- Touch screen control - no additional PC/laptop needed
- Simple, sleek, easy to learn, easy to use software
- Wi-Fi option for sending results straight via email
- Compact, space-saving design
ADAM™ CellT is based on staining mammalian cell DNA with a fluorescent dye, Propidium Iodide (PI). PI does not enter cells with intact membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained.
The ADAM™ CellT provides two kinds of staining solutions. AccuStain Solution T (Propidium Iodide stain) for the total cell counting is composed of the fluorescent dye (PI) and lysis solution. AccuStain Solution N (Acridine Orange stain) for the non-viable cell counting is composed of the fluorescent dye and PBS.
In order to measure the total concentration of cells, the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI. The process of disrupting and staining is achieved by treatment with AccuStain Solution T.
In the second solution, live cells remain intact and are not stained. Only the non-viable cells are stained and detected. After treatment, the prepared cells will be loaded into the chip. The viability will be automatically calculated in the ADAM™ CellT software after each measurement of the total cells and the non-viable cells.
ADAM calibration beads allow quick diagnosis when problems occur, which ensures accuracy of the instrument.
Certifications: ADAM™ CellT complies with 21 CFR part 11.
Ordering information: Initial order comes with a starter kit including AccuChip kit and Calibration bead.
