TaqDog 2× Green Master Mixes provide everything needed to perform PCR. Just add template and primers and then start amplifying.
- Faster preparation time
- Enhanced for hard-to-amplify targets
- Loading dye for direct gel loading
- Robust and stable for many months
Easy setup provides reliable amplification
TaqDog 2× Green Master Mixes provide everything needed to perform PCR. Just add template and primers and then start amplifying. These master mixes are based upon our popular TaqDog DNA Polymerases and includes nucleotides, buffer, and a brilliant green dye allowing for amplified samples to be directly loaded onto gels. Formulated for a 50 ul reaction size, its robust processivity makes smaller reaction volumes an economical option for labs looking to stretch their lab’s dollars. TaqDog master mixes come in two versions, premium and hot start. Our TaqDog Premium master mix is our most affordable option and provides great results in most cases. The TaqDog Hot Start master mix includes an aptamer-based hot start technology that promises better yields with no interference from primer-dimers.
Enhanced to conquer GC-rich templates
Some PCR master mixes come with a separate tube of “PCR enhancer” for more difficult templates, especially those with increased G-C nucleotide content. The additional hydrogen bonding for G-C pairs makes PCR less efficient — often resulting in weak results. Both types of TaqDog 2x Green Master Mixes also include their own unique enhancer reagents that can be added when amplification efficiency needs to be improved.