Sulfo-NHS-LC-Biotin, EZ-Link™, Pierce™
Supplier: LIFE TECHNOLOGIES MS
Synonyms:
Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
, Sulfosuccinimidyl-6-(biotinamido)hexanoate
, Sulphosuccinimidyl-6-(biotinamido)hexanoate
, Biotinamidohexanoic acid 3-sulpho-N-hydroxysuccinimide ester sodium salt
Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
- Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
- Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
- Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
- Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
- Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
- Medium length—spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
Sulfo-NHS-LC-Biotin is one of three very similar EZ-Link Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-LC-Biotin is offered in several package sizes and as complete protein labeling kits.
Caution: For Research Use Only. Not for use in diagnostic procedures. Store desiccated at -20°C.
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Formula:
C₂₀H₂₉N₄NaO₉S₂ MW: 556.59 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00078534 CAS Number: 127062-22-0 |
Specification Test Results
| Description | EZ-Link Sulfo-NHS-LC-Biotin |
| Product Type | Sulfo-NHS-LC-Biotin |
| Quantity | 100 mg |
| Product Line | EZ-Link™ |
| Cell Permeability | Cell-Impermeant |
| Chemical Reactivity | Amine |
| Reactive Moiety | Active Ester, Succinimidyl Ester, Sulfo-NHS Ester |
| Solubility | DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water |
| Label or Dye | Biotin |
| Label Type | Biotin & Analogs |
| Spacer | Mid-Length |
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