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Extracta Plus RNA Kits, Quantabio

Supplier: Quantabio
Extracta Plus RNA<br /><br />Rapid extraction and purification of high-quality total RNA from cultured cells or tissue
Total RNA yield & negligible gDNA yield.<br /> PCR assays for human or rat ?-actin were performed. A standard curve of human/rat gDNA was used to quantify the amount of gDNA contamination in the purified RNA. For all samples, gDNA was present in negligible quantities.
Effective gDNA removal from cells and tissue.<br />Total RNA was purified from A 1 x 106 HeLa cells or B 10 mg rat kidney tissue using the Extracta Plus RNA Kit. RT-qPCR assays were performed with (+RT) or without (–RT) reverse transcriptase.
Quantitative extraction of RNA from dilute cell quantities.<br />Total RNA purified from HeLa cell from 100,000 to 10 cells using the Extracta Plus RNA Kit. RNA (1.5 ?l) was used for qPCR analysis of ?-actin mRNA using qScript XLT ToughMix.
Extraction of intact high molecular weight RNA from tissues and cultured cells.<br />Total RNA purified of rat kidney or lung tissue or from 1X106 cultured HeLa cells (H) using Extracta Plus RNA Kit and amplified via RT-PCR using qScript µltra Flex Kit and repliQa HiFi Toughmix.
Typical yields from various input samples.
Extracta Plus RNA procedure.<br />This procedure can be completed in just 25 mins, resulting in ready-to-use total RNA applicable for sensitive downstream applications such as RT-qPCR and NGS.
Extracta Plus RNA<br /><br />Rapid extraction and purification of high-quality total RNA from cultured cells or tissue
Total RNA yield & negligible gDNA yield.<br /> PCR assays for human or rat ?-actin were performed. A standard curve of human/rat gDNA was used to quantify the amount of gDNA contamination in the purified RNA. For all samples, gDNA was present in negligible quantities.
Effective gDNA removal from cells and tissue.<br />Total RNA was purified from A 1 x 106 HeLa cells or B 10 mg rat kidney tissue using the Extracta Plus RNA Kit. RT-qPCR assays were performed with (+RT) or without (–RT) reverse transcriptase.
Quantitative extraction of RNA from dilute cell quantities.<br />Total RNA purified from HeLa cell from 100,000 to 10 cells using the Extracta Plus RNA Kit. RNA (1.5 ?l) was used for qPCR analysis of ?-actin mRNA using qScript XLT ToughMix.
Extraction of intact high molecular weight RNA from tissues and cultured cells.<br />Total RNA purified of rat kidney or lung tissue or from 1X106 cultured HeLa cells (H) using Extracta Plus RNA Kit and amplified via RT-PCR using qScript µltra Flex Kit and repliQa HiFi Toughmix.
Typical yields from various input samples.
Extracta Plus RNA procedure.<br />This procedure can be completed in just 25 mins, resulting in ready-to-use total RNA applicable for sensitive downstream applications such as RT-qPCR and NGS.
Extracta™
95214-010 95214-050 95214-050 95214-010
76492-574EA 78.57 USD
76492-574 CA76492-574 76492-576 CA76492-576
Extracta Plus RNA Kits, Quantabio
Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents
Rapid extraction and purification of high-quality total RNA from cultured cells or tissue.

  • Rapid protocol – from cells or tissue samples to high-quality total RNA in just 25 minutes
  • Streamlined gDNA removal – novel DNA removal column eliminates gDNA contamination and need for DNase treatment
  • Extraction of low input quantities – ability to extract RNA from as little as 10 cells
  • Downstream compatibility – ideal for applications such as RT-qPCR and next generation sequencing

The Extracta Plus RNA kit provides rapid extraction and purification of total RNA from cultured human or animal cells and from tissue samples. The Extracta Plus spin column workflow enables simultaneous processing of multiple samples in 25 mins to yield purified total RNA suitable for a range of downstream applications. The kit includes specially designed Extracta Plus DNA removal columns for effective removal of genomic DNA contamination, providing a convenient solution for applications that are sensitive to low amounts of DNA contamination such as RT-qPCR and NGS.

The efficient, reproducible extraction process allows total RNA extraction from as little as ten cells input quantity.

The Extracta DNA removal column achieves effective gDNA removal without a separate DNase treatment. Quantification of residual gDNA by qPCR demonstrates negligible gDNA within the total RNA extracted from cultured cells or tissues.

Successful extraction of intact high molecular weight RNA was demonstrated by RT-PCR of long targets, ideal for cloning or long range sequencing applications.
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