Pre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR with high sensitivity and reproducibility.
- Preformulated, predispensed, single-dose, ambient-temperature-stable beads ensure greater reproducibility between reactions, minimize pipetting steps, and reduce the potential for pipetting errors and contamination.
- Optimized as a one-tube, one-step RT-PCR reaction for both cDNA synthesis and PCR.
- Each lot of RT-PCR Beads is function tested for its ability to generate highly specific PCR products to ensure lot-to-lot reproducibility
- For highly reproducible one-step reverse transcription-PCR.
Ready-To-Go RT-PCR Beads are stable at room temperature and designed for performing single-tube one-step reverse transcription-PCR. Each room-temperature-stable bead contains M-MuLV Reverse Transcriptase, RNase Inhibitor, buffer, nucleotides, and Taq DNA Polymerase. The only additional reagents required are water, template RNA, and primers. The reagents are optimized for full-length cDNA synthesis to >7.5 kb and optimal sensitivity from PCR.
The Ready-To-Go Bead format significantly reduces the number of pipetting steps, thereby increasing reproducibility of the RT-PCR technique and minimizing risk of contamination and RNA degradation. Ready-To-Go RT-PCR Beads are provided in either thin walled 0.5 ml or 0.2 ml tubes compatible with most thermocyclers. The 0.2 ml tubes come assembled in a 96-well (8 × 12) plate format that allows individual strips of eight tubes to be easily removed. This flexibility allows use of either the entire 96-well plate, strips of eight or individual 0.2 ml tubes. Each package of Ready-To-Go RT-PCR Beads contains: RT-PCR beads, control reactions and pd(N)6 and oligo(dT) cDNA primers.
Delivery information: Kit contents Room-temperature stable beads containing buffer, dATP, dCTP, dGTP, dTTP, approximately 2.0 units of Taq DNA polymerase, M-MLV reverse transcriptase, RNAguard (porcine) and stabilizers (including RNase/ DNase- Free BSA). When a bead is reconstituted to 50 µl, the concentration of each dNTP is 200 µM in 10 mM Tris-HCl (pH 9.0 at room temperature), 60 mM KCl, and 1.5 mM MgCl2
Caution: Storage conditions Ambient in pouch with desiccant
