PaqCI, New England Biolabs

Supplier: New England Biolabs
R0745S R0745L R0745L R0745S
76470-742EA 462 USD
CA76470-742 CA76470-740 76470-742 76470-740
PaqCI, New England Biolabs
Nucleic Acid Reagents Restriction Enzymes
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(⁴/₈).

  • Useful in Golden gate assembly - 7 bp cutter leads to less domestication problems
  • PaqCI is an isoschizomer of AarI that effectively cuts to completion (unlike AarI)
  • Requires 2 or more sites for cleavage. PaqCI Activator supplied to improve cleavage

It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's CutSmart buffer and is heat inactivated.
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