Monarch® Genomic DNA Purification Kits, New England Biolabs
Supplier: New England Biolabs (NEB)
Quickly and easily purify high quality, high molecular weight gDNA from multiple sample types, all with one kit!
- For use with a broad range of sample types, such as cells, blood, and tissues (including fatty and fibrous tissues)
- Also works with tough to lyse samples (e.g., bacteria, yeast)
- Suitable for clinically-relevant samples (e.g., saliva, cheek)
- Obtain excellent yields of highly-pure DNA
- Extremely low residual RNA contamination (typically <1%)
- Isolates high molecular weight gDNA (peak size typically ≥50 kb)
- Use DNA directly in downstream applications, including PCR, qPCR and NGS
- Fast, user-friendly protocols with short lysis times and minimal hands-on time
- Can also be used to clean up previously extracted gDNA
- Includes RNase A
- Kit components available separately
The Monarch® Genomic DNA Purification Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues.
Additionally, bacteria and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Protocols are also included to enable purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA.
Purified gDNA has high quality metrics, including A260/A280 >1.8 and A260/A230 >2.0, high DIN scores and minimal residual RNA.
The purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has a peak size of >50 kb, making this kit an excellent choice upstream of long-read sequencing platforms.
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