NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs), New England Biolabs
Supplier: New England Biolabs (NEB)
NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina® sequencing and includes the NEBNext EM-seq™ Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification.
- Optimized for the EM-seq workflow
- Compatible with bisulfite sequencing workflows
- Addresses the 'barcode hopping' issue seen with some Illumina sequencing instruments
- Increases sample identification specificity with unique dual index primer pairs
The index primers are provided as pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal, or in individual vials. Although optimized for the EM-seq™ workflow, the NEBNext EM-seq™ Adaptor is also compatible with bisulfite sequencing workflows.
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