Mix-n-Stain™ Nanobody Labeling Kits, Biotium

Supplier: Biotium

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76423-378EA 149.85 USD
76423-378 76423-366 76423-388 76423-368 76423-396 76423-374 76423-384 76423-376 76423-386 76423-392 76423-370 76423-380 76423-394 76423-372 76423-382 76423-390
Mix-n-Stain™ Nanobody Labeling Kits, Biotium
Protein Linking and Labeling Systems
The simplicity and convenience of Mix-n-Stain™ labeling of nanobodies with fluorescent CF® dyes or biotin.

  • Rapid, simple, covalent conjugation of Nanobodies® (also called camelid single variable or VHH domains)
  • No purification required after labeling
  • Reaction takes only 30 minutes, with minimal hands-on time
  • Choice of seven next-generation CF® dyes with superior brightness and photostability, or biotin
  • Compatible with common stabilizers

Mix-n-Stain™ Nanobody labeling kits allow to rapidly label 5 to 20 µg or 20 - 50 µg Nanobody® (also called camelid single variable or VHH domains) with one of Biotium's next-generation CF® dyes or biotin. Simply mix your Nanobody® with the reaction buffer and optimally formulated dye provided, followed by a 30 minute incubation and addition of the provided stop buffer. Any remaining free dye or other label is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. After the reaction, the Nanobody® will be labeled with an average of 1 to 2 dye molecules per Nanobody® molecule. Mix-n-Stain™ labeling is covalent, so labeled nanobodies can be used for multiplex staining without transfer of dye or biotin between proteins.

These kits feature a choice of Biotium’s next-generation CF® dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Biotium also offers kits for labeling IgG antibodies with CF® dyes and other labels.

Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific. Cy Dye is a registered trademark of GE Healthcare.

Ordering information: These kits are optimized for a single labeling reaction with 5 to 50 µg Nanobody®. Mix-n-Stain™ kits are not designed to be used for multiple labeling reactions.

Delivery information: Kit components: Lyophilized reactive dye or biotin, reaction buffer, BSA solution, quenching buffer, ultrafiltration vial.
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