The Monarch® RNA Cleanup Kit (50 µg) enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.
- Elute in ≥6 µl for concentrated RNA
- 70 to 100% RNA recovery, even with inputs <20 ng
- Efficiently purify RNA ≥25 nt (a simple modification enables purification of RNA ≥15 nt)
- Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
- Simplified workflow with a single wash buffer
- Unique column design prevents buffer carryover and elution of silica particulates
- Columns and buffers are available separately
- Purified RNA is ready for use in a wide variety of downstream applications
The Monarch® RNA Cleanup Kit (50 µg) rapidly and reliably purifies up to 50 μg of concentrated, high-quality RNA (>25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥15 nts).
Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.
Monarch® RNA cleanup kits are also available for 10 µg and 500 µg binding capacities. Columns and buffers are also available separately for convenience.
