Premier® EHEC Kit, Meridian Bioscience

Supplier: Meridian Bioscience

Premier EHEC test

Premier EHEC test

Premier®

608096

76317-618EA 1711.87 USD

76317-618

Premier® EHEC Kit, Meridian Bioscience

Assays ELISAs

The Premier® EHEC test is a rapid in vitro microwell EIA for the detection of Shiga toxins I and II (Verotoxins) in stool specimens, broth cultures, from individual colonies or colony sweeps of agar plates. This test is intended for use as an aid in the diagnosis of enterohemorrhagic E. coli (EHEC) infections.

This test utilizes monoclonal anti-Shiga toxin capture antibodies absorbed to microwells. Four diluted samples are added to the wells and incubated at room temperature. A wash is performed to remove unbound material. Polyclonal anti-Shiga-like toxin antibodies are added for detection and incubated at room temperature. Another wash is used to remove unbound antibody. Enzyme conjugated anti-IgG polyclonal antibody is added and incubated at room temperature.

If toxin is present, a reactive antibody-enzyme complex is formed. After washing to remove unbound conjugate, substrate is added and incubated for 10 minutes at room temperature. Color develops in the presence of bound enzyme. Stop solution is added and the results are interpreted visually or spectrophotometrically.

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