5(6)-Carboxyfluorescein ≥95% (by HPLC) fluorescent pH indicator

Supplier: Biotium

Synonyms: 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-ar-carboxylic acid5(6)-FAM

51013
89139-248EA 66.84 USD
89139-248
5(6)-Carboxyfluorescein ≥95% (by HPLC) fluorescent pH indicator
5(6)-Carboxyfluorescein

5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.


  • λExλEm (pH 9) = 492/514 nm
  • ε (pH 9) = 82,000
  • Cell permeability: Membrane impermeant
  • Cellular localization: Fluid phase tracer
  • pKa 6.5
  • Colors: Green
  • Yellow orange solid soluble in pH>6 water
  • Store at 4°C and protect from light, especially in solution

Formula: C₄₂H₂₄O₁₄
MW: 752.63 g/mol
MDL Number: MFCD00151081
CAS Number: 72088-94-9

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