HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥99% (by alkalimetry), Molecular Biology Grade, Calbiochem®

Supplier: MilliporeSigma

Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acidN-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acidHEPES free acid

391340-25GM 391340-25GM 391340-250GM
80502-684EA 397.15 USD
CA80502-686 80502-684 80502-686
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥99% (by alkalimetry), Molecular Biology Grade, Calbiochem®
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

A zwitterionice N-substituted aminosulfonic acid buffer.


  • Useful in the pH 6.8 to 8.2 range
  • Has a pKa of 7.48 at 25 °C

Formula: C₈H₁₈N₂O₄S
MW: 238.31 g/mol
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
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Specification Test Results

Purity ( by alkalimetric assay) ≥99%
DNase, RNase, and protease None detected
Heavy metals ≤0.0005%

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