The P-97 Flaming/Brown type micropipette puller is ideal for fabricating micropipettes, patch pipettes and microinjection needles.
- Programmable air pressure
- Memory storage for up to 100 programs
- Write protection and date stamp for each program
- Two cooling modes: time and delay
- Consistent and reliable electrodes with tip diameters less than 0.1 µm
This environmental chamber is designed to minimize the effect of changing humidity on the reproducibility of pulled pipettes. The metal jaws that clamp the heating filament have also been redesigned to minimize heat retention. A gas delivery mode switch provides for extended cooling for large diameter and multi-barreled glass. A spring-loaded clamping mechanism has been added for easier loading of glass. A vacuum fluorescent display has been added that allows easy viewing. Ramp test - self test for establishing program heat settings when a new filament or glass is introduced Vacuum fluorescent display. Quality control, SEM photograph of a tip pulled with each puller; criterion is tip measurement less than 0.1 µm and typically is ~0.06 µm.
Software improvements on the P-97 include a display of the total heat-on time to assist in program development and troubleshooting. Up to 100 programs can now be written and stored in memory, which makes the P-97 suitable for multiple users. These programs can now be write-protected, adding security to prevent programs from being changed or altered inadvertently. The display shows the last date and time the program was written or edited. In addition, the air pressure is included as a programmable parameter.
Used in patch pipettes, sharp electrodes, pronuclear injection, zebrafish injection, insect egg injection and aspiration pipettes.
