2X SDS-PAGE Sample Buffer
- 0.125 M Tris-HCl, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromphenol blue, pH6.8
- aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers
- The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins.
2X SDS-PAGE Sample Loading Buffer is suitable for laboratory involved in protein biochemistry. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris-HCl, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromphenol blue. The pH of this solution is 6.8. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
