Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component System), Rockland Immunochemicals

Supplier: Rockland Immunochemicals
FEMTOMAX-020
RLFEMTOMAX-020EA 174.48 USD
RLFEMTOMAX-020
Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component System), Rockland Immunochemicals
Assays Enzyme Assays
Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component System)

  • use in microwell or membrane applications by mixing 1 mL of Reagent A with 1 mL of Reagent B
  • Chemiluminescent HRP Substrate is a highly sensitive detection reagent

FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP).  FemtoMax™ is designed for both western blotting and enzyme immunoassay (EIA) use.  FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras.  Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of detection reagents and re-probed. Use the same blotting conditions for FemtoMax™ as you would when using Amersham ECL Plus™ Substrate or Pierce SuperSignal® West Femto Substrate.

Prepare FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B).  Mix well.  Protect from light.  Larger or smaller volumes of the substrate can be prepared by mixing components at the same 1:1 ratio.  FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is a highly sensitive detection reagent.  Always carefully optimize all components of individual assays (antigens, antibodies, conjugates…) to minimize background reactivity associated with non-specific binding.
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