EnzyChrom™ Nitric Oxide Synthase Inhibitor Screening Kit, BioAssay Systems

Supplier: BioAssay Systems
EINO-100
76236-132EA 662.68 USD
76236-132
EnzyChrom™ Nitric Oxide Synthase Inhibitor Screening Kit, BioAssay Systems
Assays Enzyme Assays
HTS for inhibitor screening and evaluation of nitric oxide synthase inhibitors.

  • High-throughput, homogenous “mix-incubate-measure” type assay
  • Can be readily automated on HTS liquid handling system.
  • Rapid and reliable
  • Can be completed in less than 3 hours if assay performed at 37 °C.
  • Method: OD540 nm

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO is the oxidation product of arginine by nitric oxide synthase (NOS) and is involved in host defense development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in research and drug discovery. EnzyChrom™ Nitric Oxide Synthase Inhibitor Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. Samples: Nitric Oxide Synthase.
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