Bis(2-hydroxyethyl)amino tris(hydroxymethyl)methane (BIS-TRIS) ≥99%, white powder, Cell Culture Grade
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Warning
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Synonyms:
2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane (BIS-TRIS), 2-(Bis(2-hydroxyethyl)amino)-2-(hydroxymethyl)propane-1,3-diol, 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.
- Grade : Cell culture reagent
- Purity :≥99%
- Suitability : Suitable for cell culture
- Key Applications : Used in electrophoresis buffer
- Application Areas : Molecular Biology
- Product Type : Biochemicals
- Biochemical Category : Buffers
- Extinction Coefficient : (E1%) EM (water): 259 nm = 10; 277 nm = 10 (Lit.)
- Presentation : White Powder
- pH : 9 - 10 (1% aq soln); Useful pH Range: 5.8-7.2 (Lit.)
- pKa : 6.5 (25 °C)(Lit.)
- Detection Method : FTIR
- Solubility : Soluble in water (1 M - clear, colorless solution).
- Sterilization of Solutions : For sterilization of aqueous solutions, MP Biomedicals recommends sterile filtration with a 0.2 µm filter; however, the product is typically stable for autoclaving.
Formula:
C₈H₁₉NO₅ MW: 209.24 g/mol Storage Temperature: Ambient |
MDL Number:
MFCD00002853 CAS Number: 6976-37-0 |
Specification Test Results
Identity Test | Passes |
Purity | Purity |
FTIR | Conforms to Standard C |
Melting Point | 101 - 106°C |
pH (1% aq soln) | 43353 |
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