The three pNLF1 Vectors create N- or C-terminal fusions of NanoLuc luciferase and your protein of interest using a multiple cloning site (MCS). Insert your protein on the N or C terminus of NanoLuc luciferase or the N terminus of secreted NanoLuc enzyme.
- Generate N- or C-Terminal Fusions with NanoLuc(R) Enzyme or N-Terminal Secreted NanoLuc(R) Luciferase
- Use traditional cloning to insert protein of interest into the multiple cloning site (MCS)
- Bright NanoLuc(R) reporter enables endogenous expression levels of fusion proteins for biologically relevant results
- Choose from transient transfection or select for stable expression using hygromycin
The small size (19.1kDa) and extreme brightness (about 100-fold brighter than either firefly [Photinus pyralis] or Renilla reniformis) of NanoLuc luciferase (Nluc) make it an ideal protein fusion partner. NanoLuc fusion proteins can be used in a variety of applications including: reporters of protein stability, probes for bioluminescent cell imaging (BLI) or as the donor signal in bioluminescent resonance energy transfer (BRET) applications for protein:protein or protein:small-molecule interaction studies. The NanoLuc protein fusion vectors enable simple generation of N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. The pNLF Vector series uses using traditional cloning with a multiple cloning site (MCS) to generate N- or C-terminal fusions to the full-length Nluc protein with the pNLF1-N or pNLF1-C [CMV/Hygro] Vectors or attach secreted Nluc to the N-terminus of the protein of interest with pNLF1-secN [CMV/Hygro] Vector. Also make NanoLuc protein fusions with the pF Vector series: Generate N- or C-terminal Nluc fusion proteins using the Flexi Vector Cloning System—a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, that provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence.
![pNLCoI4 [luc2-P2A-nlucp/PGK/Hygro] vectors](https://us.vwr.com/stibo/low_res/std.lang.all/52/48/44105248.jpg "pNLCoI4 [luc2-P2A-nlucp/PGK/Hygro] vectors")
![pNLCoI4 [luc2-P2A-nlucp/PGK/Hygro] vectors](https://us.vwr.com/stibo/grid/std.lang.all/52/48/44105248.jpg)
