Fast RNA detection with these easy-to-use RNA LAMP (RT-LAMP) Master Mixes
- Isothermal Amplification: Facilitates running amplification reactions with simplified instrumentation
- Master Mix Format: Streamlines reaction setup while reducing potential handling errors
- Simplified Optimization: Focuses optimization on the two critical reaction parameters - primer design and reaction temperature
- Lyophilization-ready: Avoids redesign of assays to remove components known to inhibit lyophilization – all components are lyophilization-compatible
The LavaLAMP® RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which the RNA target is first converted into cDNA and then that cDNA acts as template for additional LAMP, all using a single DNA polymerase. By combining the strong activity of this enzyme with an optimized master mix formulation, we’ve produced an easy-to-use system that enables fast, sensitive detection of specific RNA targets.
Most LAMP systems are available as multi-component kits that require optimization of many parameters such as MgSO4, betaine, and enzyme concentrations as well as reaction temperature, primer design and primer concentration. The LavaLAMP RNA Master Mix greatly simplifies reaction optimization by providing a “pre-optimized reaction formulation” allowing you to focus your optimization experiments on the two most important reaction parameters – RNA LAMP primer design and reaction temperature.
Certifications: For research use only. Not for human or diagnostic use.
Ordering information: Both LavaLAMP™ RNA Master Mix kits contain: LavaLAMP RNA Master Mix, RNA Positive Control LAMP Primer Mix, and RNA Positive Control. The LavaLAMP RNA Master Mix with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA.
Caution: Licensing information: Lucigen is a fully licensed provider of LAMP reagents for research use. Patents WO 00/28082, WO 01/34790, and WO 01/77317 regarding the LAMP method are owned by the Eiken Chemical Co. Ltd. LavaLAMP™, OmniAmp® and Bst Polymerase, Exonuclease minus are sold by Lucigen under license for use in LAMP for research use only. The products may not be used for LAMP-based human or diagnostic purposes without obtaining a license from Eiken. US Patent 8093030 for LavaLAMP and OmniAmp is owned by Lucigen Corp.
It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties. If the purchaser is not willing to accept these use limitations, Lucigen Corporation is willing to accept return of the product for a full refund.
