CSM-Leu is a Single dropout formulation of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.
- Complete Supplement Mixture
- Time-tested dropout supplement
- For virtually all strains of S. cervisiae
- Vigorous cell growth
CSM-Leu is a Single dropout formulation of Complete Supplement Mixture (CSM) of amino acids for S
CSM-Leu is a dropout supplement for virtually all strains of S. cerevisiae and allows to select for auxotrophic requirements and transformants. Cells grow vigorously in CSM-Leu supplemented medium which is easily made by adding the CSM-Leu powder to DOB of DOBA Base Medium and autoclaving.
Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM-Leu formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7g DOBA powder and 0.69 g of CSM-Leu to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. After cooling to 50 °C plates were poured.
cerevisiae growth media.
